- Milo Thatch: At last, the expedition is underway. The Journal speaks of ancient guardians, protectors of the gateway to Atlantis. Hopefully, that's all just myth and superstition.
- Commander Lyle Tiberius Rourke: Thatch! I won't sugarcoat this. We've got a crisis on our hands! We're under attack and the guns are malfunctioning. Find Audrey and get her to repair 'em!
- Preston B. Whitmore: Hello Milo, name's Whitmore. Your grandfather and I go way back. When he died, he asked me to train you for the dangerous journey ahead. So, before I let you go, you'll have to complete a few tasks. The first task is simple, just copy my moves. We'll start with jumping.