A chocolate bar House eats in the hospital chapel is Cadburys Dairy Milk, an English chocolate company that Hugh Laurie did voiceovers for.
Hugh Laurie's character prescribes cigarettes to a patient. On a comedy sketch show, Laurie once played a patient whose doctor prescribes cigarettes.
The section of the opening theme song sequence where House is walking towards the camera with Cameron, Chase and Foreman is taken from this episode at around 38:30.
When Sister Augustine first moves into the cleanroom, neither Cameron, Chase, or Foreman wear facemasks, which are vital to maintaining the sterile environment.
Dr. House plays "Silent Night". Hugh Laurie is an accomplished piano player.