In a nod to Gargoyles (1994), when Manny transforms, he grows a pair of Gargoyle-themed wings as the Gargoyles theme song is heard. He uses the phrase "I Live Again", which is similar to "We Live Again" from the aforementioned show. Additionally, Manny's voice actor, Keith David, also voiced the main character Goliath in that series.
Features four former cast members from the sitcom Community (2009): Danny Pudi, Jim Rash, Paget Brewster, and Keith David
When airing as 3 separate episodes, each part had been titled "A Tale of Three Webbys!", "The Lost Library of Isabella Finch", and "Tale's End" respectively, the latter of which refers to it being the series' final episode.
It is revealed that Darkwing is far too ignorant to realize that Fenton is Gizmoduck and the only known character incapable of figuring it out.
The design of the mechanical crab prop from Funzo (which F.O.W.L. also had security cameras installed in) is based off Sebastian from The Little Mermaid (1989).