When Fletcher sees the double rainbow in the A.N.T. Farm, he says "whoa, a double rainbow! So intense... what does it mean?!" This is most likely a reference to a popular internet video released around the same time. The video is titled "Yosemitebear Mountain Double Rainbow 1-8-10" (1-8-10 signifying the date it was posted, January 8th, 2010) The video is more well known as Double Rainbow Guy.
This episode features the first mention of Fletcher's Grandma Dottie, who would later become a recurring gag on the show. After Gibson walks in asking if his llama bite looks infected, an easel can be seen behind him. On it is a large pad of paper, where Fletcher has written a letter to Grandma Dottie, thanking her for a homemade sweater and five dollars, and hoping her case of gout is healing.